Synchronised heads the 48 remaining entries for Saturday's John Smith's Grand National at Aintree.

Jonjo O'Neill's Gold Cup winner will bid to become the first horse to lift that crown and follow up in the National since the legendary Golden Miller, as long as he comes throughout his final piece of work unscathed.

O'Neill has left in another of his Festival winners in Kim Muir winner victor Sunnyhillboy, as well as Arbour Supreme. Last year's National hero Ballabriggs is one of two big chances for Donald McCain who also runs Weird Al.

Calgary Bay, Neptune Collonges and Alfa Beat are others towards the head of the weights to stand their ground.

Despite the absence of Quel Esprit, Apt Approach and one-time favourite Prince De Beauchene, Willie Mullins still has a strong team, led by On His Own, the mount of Ruby Walsh.

The Midnight Club, sent off favourite last year and Quiscover Fontaine, placed in the Irish National last year could also run for Ireland's champion trainer.

Junior, Seabass, Chicago Grey, Treacle, Organisedconfusion, Cappa Bleu and State Of Play are among the others all still in the mix.

As expected, Burton Port and Midnight Chase were taken out.